Ultrastructural alterations in macrophages after phagocytosis of acrylic microspheres
Identifieur interne : 000B03 ( Main/Exploration ); précédent : 000B02; suivant : 000B04Ultrastructural alterations in macrophages after phagocytosis of acrylic microspheres
Auteurs : Peter Edman [Suède] ; Ingvar Sjöholm [Suède] ; Ulf Brunk [Suède]Source :
- Journal of Pharmaceutical Sciences [ 0022-3549 ] ; 1984-02.
English descriptors
- KwdEn :
- Macrophages, peritoneal—cultured from mice, effect of phagocytosis of microparticles, ultrastructural cellular alterations, Microspheres—polyacrylamide, phagocytosis by cultured mouse peritoneal macrophages, ultrastructural cellular alterations, Phagocytosis—microparticles, by cultured mouse peritoneal macrophages, ultrastructural cellular alterations.
Abstract
The effect of microparticles on the survival of cultured mouse peritoneal macrophages was investigated using doses of 0.01‐0.1 mg of lyophilized particles/ml of medium and 5 ± 105 cells, corresponding to ∼4000–40,000 particles per cell. The lowest dose did not significantly change the survival time as compared with the controls, while ∼75% of the cells were lost during the first 48 h on exposure to the highest dose. High doses of particles induce cellular damage. The morphology and stability of the lysosomal apparatus was followed with electron microscopy, acid phosphatase cytochemistry, and acridine orange uptake. Alteration of the lysosomal vacuome was characterized by a greatly enhanced rate of autophagocytosis, the formation of huge secondary lysosomes containing microparticles, and labilization of the vacuome with loss of acidity and a tendency to leak acid phosphatase into the cell sap.
Url:
DOI: 10.1002/jps.2600730204
Affiliations:
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The lowest dose did not significantly change the survival time as compared with the controls, while ∼75% of the cells were lost during the first 48 h on exposure to the highest dose. High doses of particles induce cellular damage. The morphology and stability of the lysosomal apparatus was followed with electron microscopy, acid phosphatase cytochemistry, and acridine orange uptake. 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